Dr.Pallav Kaushik Deshpande

Hobbies & Passion

• Research Experience: Patent : 202021001557 Title: ANTI MALARIAL MOLECULES OF NATURAL AND SYNTHETIC ORIGIN WITH DRUG CARRIER FOR PREGNANT SUBJECT.  
• Women Scientist under UGC from June 2016 working in Department of Biotechnology ,Barkatullah University,Bhopal. Tittle of project : Neurogenerative evaluation of Celastrus peniculatus and Bacopa monnieri through drug delivery system on cultured hippocampal primordial cells subjected to neurotoxin .
• Ph.D : Ph.D in Biotechnology, under the guidance of Dr.Ragini Gothalwal, Deptt. Biotechnology and co-guidance of Dr.A.K Pathak, Deptt. Pharmacy, B.U Bhopal. Title of Thesis: Development of Noval Drug Delivery System for Antimal Drugs From Natural and Synthetic Origin.
• Studies Undertaken: • Biochemical Analysis of Major Components of Synovial Joints. • Basic Knowledge of Animal Tissue Culture. • Cultured Chondrocytes Cells in Alginate Beads. Training Experience: Name of the Industry: M\S Genni, Bangalore Techniques Learnt: • Basic Molecular Biology Techniques. • Plasmid Isolation. • Agarose Gel Electrophoresis. • DNA Isolation. • PCR • Rt-PCR. Biotech. Consortium India Limited (BCIL) Sponsored Six Months Training, at Dabur Research Foundation. Under the guidance of Dr.Manish Diwan, Manager, Experimental Oncology Department. IN VITRO Techniques. A. Basic Cell Culture Techniques: • Handling • Maintaining the Culture. • Sub-Culturing\ passage. • Harvesting • Reducing • Freeze Down Preparation \Cryopreservation Types of Cell Line Handled. Primary Cultures: I.Primary Culture of Dentritic Cells: Primary cultures of stem cells isolated from Bone marrow preparation are maintained and studied their propagation into dentritic cells. II.From Spleenocytes preparation: Purification and propagation of T-cell. Secondary Culture: Cancerous cell lines: Handling and maintenances of COLO and MIAPPACA cell line. B. FAC`S: Harvesting, Cell staining with Fluorescence Labeled Monoclonal Antibodies: Primary dendritic cells were treated with various phytochemicals, peptides, compounds of marine origin for the screening of their potential as the Immunomodulatory and Anti- inflammatory properties. • Some cells were harvested from the primary culture which is not treated with any chemical compound, stained with fluorescent dye attached to monoclonal antibodies; cells were then subjected to Flow Cytometry (fluorescence activated cell sorter) which analyses large population of cell for the amount of receptor on their surface as the level of fluorescence. • Some cells from the primary culture were subjected to various chemical treatments periodically, then stained with fluorescent dye attached to monoclonal antibodies and then subjected to Flow Cytometry. • Preliminary known to data acquisition and data analysis using Cell Quest software. • Machine Used: FAC`S calibrator with four filters. C. Enzyme Linked Immunosorbent Assay (ELISA) • Used commercially available kits for detecting cytokines examples, IL-6, IL-10, IL-12, RANTES, IFN-γ, TGF-β, and TNF-α . • These cytokines were secreted by cell population when exposed to various chemical compounds. • Through the analysis of results obtained from Elisa, it is calculated which chemical is responsible for the secretion of which cytokine, in this way surface reactor which facilitates the secretion can be traced. • Reference curves generated with linear regression curves fitting with r2 value >0.98. • Hands on experience of using Graph pad prism for data analyses and interpretation of result. D. Cytotoxic Assay (MTT Assay): MTT is yellow water soluble tetrazolium dye that is reduced by live cells to a purple formazen product that is insoluble in aqueous solution. This assay is performed to study the cytotoxicity of the drugs. IN VIVO Techniques: A. Identification and handling of small experimental animals like Rat and Mice. B. Administration of Drugs / cancerous cell lines in experimental animals through various routes of administration. • Sub-cutaneous • Intra-veneous • Intra-muscular • Intra peritoneal • Oral Hands on experience in Sub-cutaneous and Intra-veneous route of administration. C. Dissection of animal to remove various vital and desired organs for In vitro culturing. • Spleen for spleenocytes preparation for T-cell purification and proliferation. • Femur bone for stem cell preparation for dentritic cells propagation. • Lymph nodes for the study of migration of fluorescence labeled cells injected through Sub-cutaneous route. D. Necropsy performed on experimental animals which includes following steps; • Exsanguination of animal. • Gross examination of internal anatomy of animal. • Collection and preservation of various organs and glands for pathological examination. E. Blood with-drawl by capillary method for hematological analysis of experimental  animals depending upon their body weight. F. Migration assay of C.F.S.E labeled T-cells in lymph nodes of lived mice. G. Study of various type tumor in nude mouse, migration of malignant cells in a living model. Laboratory Skills: Various other techniques learned . • Agarose Gel Electrophoresis. • PAGE • Thin layer chromatography. • Basic microbiological assays. • Molecular biological techniques. • Biochemical analysis of various biological compounds (viz. DNA, RNA, protein carbohydrates). • BOD and COD estimation. • Isolation of DNA ,RNA & Proteins form animal tissue. • Basic plant tissue culture. • Animal tissue culture techniques. Computer Knowledge: Completed a certificate course. • Microsoft office • Microsoft coral draw • Adobe Photoshop • Graph pad prism. REVIEWER IN
•  Bioinfo publication
• Premier Publishers
• International Journal of agricultural sciences
•  International Journal of Biotechnology
•  International Journal of tropical disease and health
• Registered Resource person in SREJAN. Successfully  evaluated the various scientific models and projects for  2017-18-19 and 2020.